The induced pluripotent stem cells (iPSCs-DSs) express pluripotency-associated genes. (A) The total gene-expression levels of OCT4, SOX2, KLF4, and c-MYC were determined by using quantitative reverse transcription polymerase chain reaction (RT-PCR) in iPSCs-DS and hESCs. The differences in the expression levels of H7 hESCs and iPSCs-DS were not significant. The results are reported as mean ± SEM (n = 3). (B) The endogenous (Endo) and total (Total) expression levels of OCT4, SOX2, KLF4, and c-MYC were analyzed with semiquantitative RT-PCR and compared with parental human dermal fibroblasts. β-Actin (ACTB) was used as the positive control.
Mou et al. Stem Cell Research & Therapy 2012 3:14 doi:10.1186/scrt105